Getting Pregnant with Frozen Oocytes:
Frozen Embryo Transfer Method

All frozen oocytes are thawed and micro-fertilized, the embryos are frozen, and thawed embryos are transplanted in the next cycle.

Frozen Embryo Transfer Method


  • Since microinsemination is performed at one time, the total cost of microinsemination will be lower.
  • Only one semen collection is needed.


  • With two freezing cycles, the pregnancy rate is slightly decreased.
  • Increased cost of freezing and thawing due to extra freezing cycle.

Schedule Summary

Thawed oocyte & frozen embryo

Anytime Plan decision with doctor Book a day for microfertilization
〔Reservation required〕 Oocyte thawing, microinsemination
3-5 Days after fertilization Freezing embryos

Thawed Embryo Transfer

Shortly before menstruation Make a plan with the doctor
Before the 2nd day of menstruation Pre-cycle ultrasound
Collect medication
12th day of menstruation USG Endometrium Check
15-17th day of menstruation Embryo Transfer
27th day of menstruation Pregnancy test

Cost simulation

This assumes that 12oocytes (4cryotops) are thawed, micro-inseminated and given calcium ionophore treatment, then cultured to day5 after which 4embryos are frozen.
*Prepayment ¥350,000
*Prices including tax are listed based on the obligation to display the total amount. Final calculations may differ.

Item Before Tax Including Tax
Oocyte thawing (12oocytes, 4cryotops) ¥50,000 ¥55,000
12oocytes ICSI and Calcium Ionophore ¥170,000 ¥187,000
Culture to day5 ¥90,000 ¥99,000
Freezing 4blastocysts ¥44,000 ¥48,400
Total ¥354,000 ¥389,400

Afterward, using Estrana and Onecrinone mediations, there is an embryo tranfer.
*Prices including tax are listed based on the obligation to display the total amount. Final calculations may differ.

Item Before Tax Including Tax
Estrana ¥5,520 ¥6,072
Onecrinone vaginal gel ¥18,600 ¥20,460
Fee for Thawing One Embryo for Transfer ¥20,000 ¥22,000
Fee for One Embryo Transfer ¥55,000 ¥60,500
Total ¥99,120 ¥109,032

Thawed Embryo Transfer : Schedule Details

Schedule for oocyte thawing

  Please be sure your appointment is made for oocyte thawing and micro-insemination at least two days in advance by the doctor.
On the Day Day 0 Oocytes are thawed, semen is collected, micro-insemination is performed,
and hormonal medication continues.
Progesterone support begins strictly at 2PM
Calcium ionophore is also used for micro-insemination of frozen oocytes and sperm.
Semen must be submitted by 10:00.
Please bring semen within 2 to 3 hours after collection as much as possible (up to 7 hours is possible.)
For in-hospital semen collection, please come by 9:30.
After submitting the semen, please wait about 30 minutes while the lab staff confirm the presence of sperm and the plan.
The lab staff will confirm the approximate freezing period of the embryo and any limit you wish to place on frozen embryos, if applicable.
Embryo development is confirmed on the 1st and 3rd days of fertilization.
If you would like to confirm the embryo development on the 1st and 3rd days, please call us from 10:00 to 19:00 on weekdays and from 10:00 to 17:00 on weekends and holidays.
Day 3 Some embryos may be frozen on day 3
Day 5 Day 5/blastocyst freezing
Day 6  
Day 7 In special circumstances culture may continue until day 7
Day 12 Oocyte/Embryo Explanation You can have an explanation of your culture results on this day.
If you wish to transfer embryos the next month, apply for transfer with USG before the cycle.

Thawed Embryo Transfer Cycle

Before menstruation (Around one week before menstruation) Provide prepayment and documents, receive cycle medications
Menstruation Day
Call on the first day of menstruation.
Confirm negative pregnancy test.
This should be the first day when you see normal menstruation. If it occurs after 18:00, the next day is day 1. The next visit will be on approximately CD12.
2 Hormonal medication starts
12th day of menstruation
(before and after OK)
12 Visit the clinic for ultrasound of endometrium.
If the endometrium is OK, progesterone starts at 14:00(or 15:00).
The start date of progesterone is the fertilization date.
If the endometrium is 7mm or more, the thaw date will be determined two days after the next day.
If it is less than 7mm, check again in a few days.
In case of thawing cancellation, a cancellation fee will be charged from the afternoon of the day before thawing.
If the endometrium is OK at night, progesterone will start the next day.
13 ↓⇓
14 ↓⇓
3rd day after fertilization 15 Day 3 ET: Visit for procedure


Embryo transfer: Please come by 14:00(or 15:00).
16 ↓⇓
5th day after fertilization 17 Day 5 ET: Visit for procedure


Embryo transfer: Please come by 14:00(or 15:00).

18th day after fertilization 30 ↓⇓ 
Pregnancy test