Long-term Culture, Blastocyst Culture

This is the method of transfer and cryopreservation of the developed embryo and blastocyst after OPU and culturing to Day 4, 7.
We will be able to remove the embryos that stop splitting in the early term and strictly sort the good embryos.
Early embryos and post-compaction (day 3-4) embryos and blastocysts require different nutrients, so during long-term culture, change the type of culture medium on day 3.
Recently, the culture medium has been improved, and there is also a medium that can be cultured from early embryos to blastocysts.

Long-term Culture, Blastocyst Culture

Flow of Long-term Culture・Blastocyst Culture

Day 0 Oocyte Pick-Up
Conventional IVF or
ICSI Fertilization
Conventional IVF or ICSI Fertilization    
Day 1 Pronuclear Period Period or ICSI #
Pyruvic acid is the source of
energy of the early embryo
#
Culture Solution
including much pyruvic acid
#
Organization close to the
fallopian tubal fluid of people
Day 2   Period or ICSI
Period of Cell Division Period or ICSI
Day 3   Period or ICSI
Day 4 Compaction

#

Period or ICSI #
In the case of people
it will become a compaction
after 16cells and the energy
source will change to glucose
#
Culture Solution including
much glucose
#
Organization close to the uterus
Day 5 Blastocyst

Early Blastocyst
Period or ICSI
  Expanded Blastocyst Period or ICSI
  Hatched Blastocyst Period or ICSI
 

#

 

Implantation & Pregnancy