Pioneer of infertility treatment. Covering the needs of infertility from various tests 
to IVF/ICSI’s Advanced Reproductive Technologies.

〔Sumiyoshi&Ginza〕In Vitro Fertilization(IVF), Micro-Insemination(ICSI)

For in vitro fertilization(IVF), there is the standard method called Conventional IVF where it is allowed to fertilize by sprinkling the egg with a moderately adjusted concentration of sperm, and there is micro-insemination(ICSI), in which a very slim needle is used and 1 sperm is placed directly within the egg cell underneath a microscope.



Conventional IVF


This is the method of allowing the egg to fertilize by sprinkling sperm on the egg.  In the cases of fallopian tubal infertility, male infertility, anti-sperm anti-body, or when you can’t get pregnant by standard infertility treatments, or it is a case of the age factor, this will become the adaptation.



Cells called granulose cells(cumulus cells) are attached to the surroundings of the oocyte collected.  This is called the “cumulus cell oocyte complex”(COC).  For IVF, we will sprinkle an adjusted concentration of sperm on the COC(this is called, “amphipathic”).  Then, many sperm will gather around the COC, and from the head hyaluronidaze will be released and will melt the granulose cell.  After that, the sperm will pass through the “shell”(transparent body) of the egg within the granulose cell, fuse with it and fertilization is complete.



Image of Seinakadachi expansion Image sperm dissolve the zona pellucida Image that sperm arrives at the egg cell Image of fusion

From the head of the sperm hyaluronidaze is released and the granulose cell is dissolved.  Several sperm are necessary and the suitable concentration should be around 100,000⁄ml.

The sperm that dissolved the granulose cell, also dissolves the translucent body and enters the egg cell.

As soon as the first sperm arrives at the egg cell, a change occurs with the translucent body and the egg cell’s membrane, and other sperm will be denied access to enter.

The sperm that enters the egg cell along with the egg form a pronucleus and finally fuse together.




This is the method of injecting sperm into the egg by the use of a needle underneath a microscope.  The official name is the IntraCytoplasmic Sperm Injection Method(ICSI).  They are the cases of severe male infertility(can not achieve an adequate number of motile sperm with conventional IVF), and fertilization failure(will not fertilize with IVF).


Image of Immobility of Sperm Cytoplasm is sucked in by the needle. Image of injecting the sperm to egg

Even when the sperm is injected into the egg it will not fertilize.  If the tail portion of the sperm is taken with the ICSI needle, then the sperm will fail to move(immobility of sperm), and we will have to acquire fertilization.

We will take the immobile sperm with the ICSI needle and just like that pierce the egg. This time, we will secure the egg so that the polar bodies will come to the positions of 12 o’clock and 6 o’clock(in the vicinity of the polar bodies there are chromosomes so we want to keep them away from the 3 o’clock position where we will be piercing with the needle).

A small amount of the egg cytoplasm will be sucked up into the ICSI needle, and the sperm with the cytoplasm are injected into the egg.




State of Micro-insemination(ICSI)



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Oak Medical Group has been from the time we opened, supporting the many issues of women's health, building performance and trust, and not only here in Osaka, but centering in Kansai we are receiving patients from across Japan.
Especially with our infertility treatment, we are widely covering the various tests to IVF/ICSI's Advanced Reproductive Technologies.  Depending on the cause of the infertility, we are handling the treatment of each patient one by one.  If you have some concerns, please don't hesitate to consult with us.