Artificial Hatching Assistance(AHA)

This is the skill used to open a hole in the zona pellucida of the blastocyst and or embryo that is to be transplanted.

To break the zona pellucida and the cell coming out is called “hatching”, and when the cell that comes out is attached to the endometrium pregnancy is achieved. Regularly after fertilization they will naturally hatch around the fifth to sixth day, but there are situations when the zona pellucida has hardened, or the blastocyst’s power itself will not hatch. What works for these cases is AHA. There is the method of dissolving the zona pellucida with the medicine called acidic tyrode, the method of opening hole by poking it with a needle and causing friction with a glass tool, and there are handful of others, but recently by use of laser has become the mainstream method.


The blastocyst at Day 5-6 after
fertilization Enlarged,
the zona pellucida is thinning.

Hatched blastocyst

Zona pellucida Aperture Method

Zona pellucida Thinning Method