Pioneer of infertility treatment. Covering the needs of infertility from various tests 
to IVF/ICSI’s Advanced Reproductive Technologies.

〔Sumiyoshi&Ginza〕In Vitro Maturation Culture(IVM) Guidance

As for In Vitro Fertilization and micro-insemination, it will be usually done on the mature eggs, but in rare cases, it can only be done on the eggs that are not yet mature.  Even if In Vitro Fertilization or micro-insemination is done on the premature eggs they are hardly ever fertilized.  That’s where we do the In Vitro Maturation Culture on the immature eggs.

What is this In Vitro Maturation Culture(IVM) ?


○A method of allowing the maturation to occur In Vitro by culturing it for 1-2 days using a special culture serum with added hormones.
○Even when IVM is done and there are some rare eggs not fertilized.
○As for the developmental potential and fertilization potential of the matured eggs by IVM, it is the same as the ones matured in the body.
○When confirming the maturation, ICSI(Intra Cytoplasmic Sperm Injection) will be required because the granulose cells covering the egg will detach(For the eggs with granulose cells not attached, generally, they can’t be fertilized by In Vitro Fertilization).
○Since ICSI will be performed at the time when the egg has matured, it is necessary that the  sperm is collected again or cryopreserved.
○Eggs are matured, fertilized and divided by IVM will be cryopreserved and then later in the menstrual cycle, thawed and the embryo will be transferred
(Since the endometrium and the eggs have different age in days, there cannot be an embryo transplant in the period of the egg collection).
○The cost of the IVM, regardless of the number of times is ¥100,000.
¥45,000 is needed to cancel.

The prices listed are the prices without taxes.


In the following cases an IVM will be done


○When maturation does not occur even after ovulation stimulants were used
○In order to avoid ovarian hyper-stimulation syndrome caused by an overreaction to the fertility drugs, when there is no choice but to harvest the eggs while they are still immature.
○When the eggs were mostly immature as a result of the maturation of eggs is not in accordance to the size of the follicle(bag the egg is in) even though the egg has become large enough.
○When at the same time the mature eggs were collected, there were many immature eggs.


Eggs that actually had IVM

Premature eggs

The body of the egg itself is small, and the surrounding granulose cell is in a condition of being small and hard.  The pink color you are seeing is the culture serum of IVM.  As for premature eggs, even if they undergo micro-insemination they do not fertilize.


We have 2 times the chromosomes(4n=92) of normal somatic cells.  As for the mature eggs, from the meiotic division becomes a chromosome number that is decreased by half(2n=46)


Premature eggs
IVM(24˜48 hours)

Restart of Meiotic Division
Restart of Meiotic Division

Mature eggs

The eggs matured by IVM.  The granulose cell has softened and spreading out, and if we look at the egg itself, the polar body has been released which is a sign of maturation.  Matured eggs have the same fertilization potential as eggs that are matured within the body.


This is the mature egg that the egg reached in second meiotic metaphase.  With the division of the nucleus, there will be an excess number of chromosomes released from outside the cell.  The released chromosomes are known as the first polar body and is a sign of maturation.

Mature eggs
sperm n=23
Restart of Meiotic Division


Fertilized Eggs

With the entry of sperm the meiosis will complete, the “Second Polar Body” will be released and it will become a fertilized egg.

Fertilized Eggs



Rescue IVM

As for the regular case of IVM, premature eggs are collected, then will undergo a culture maturation under the condition with the granulose cell attached.

At our clinic, when micro-insemination is done, we will do the emergency procedure of “Rescue IVM” on the premature eggs on which the granulose cell had been peeled off.  Additionally, with the preculture by dbc-AMP done with our own clinic’s method, better results than conventional methods have been obtained.





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Oak Medical Group has been from the time we opened, supporting the many issues of women's health, building performance and trust, and not only here in Osaka, but centering in Kansai we are receiving patients from across Japan.
Especially with our infertility treatment, we are widely covering the various tests to IVF/ICSI's Advanced Reproductive Technologies.  Depending on the cause of the infertility, we are handling the treatment of each patient one by one.  If you have some concerns, please don't hesitate to consult with us.