As for In Vitro Fertilization and micro-insemination, it will be usually done on the mature oocytes, but in rare cases, it can only be done on the oocytes that are not yet mature. Even if In Vitro Fertilization or micro-insemination is done on the premature oocytes they are hardly ever fertilized. That’s where we do the In Vitro Maturation Culture on the immature oocytes.
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The body of the oocyte itself is small, and the surrounding granulose cell is in a condition of being small and hard. The pink color you are seeing is the culture serum of IVM. As for premature oocytes, even if they undergo micro-insemination they do not fertilize.
We have 2 times the chromosomes(4n=92) of normal somatic cells. As for the mature oocytes, from the meiotic division becomes a chromosome number that is decreased by half(2n=46)
IVM(24˜48 hours)Restart of Meiotic Division
The oocytes matured by IVM. The granulose cell has softened and spreading out, and if we look at the oocyte itself, the polar body has been released which is a sign of maturation. Matured oocytes have the same fertilization potential as oocytes that are matured within the body.
This is the mature oocyte that the oocyte reached in second meiotic metaphase. With the division of the nucleus, there will be an excess number of chromosomes released from outside the cell. The released chromosomes are known as the first polar body and is a sign of maturation.
With the entry of sperm the meiosis will complete, the “Second Polar Body” will be released and it will become a fertilized oocyte.
As for the regular case of IVM, premature oocytes are collected, then will undergo a culture maturation under the condition with the granulose cell attached.
At our clinic, when micro-insemination is done, we will do the emergency procedure of “Rescue IVM” on the premature oocytes on which the granulose cell had been peeled off. Additionally, with the preculture by dbc-AMP done with our own clinic’s method, better results than conventional methods have been obtained.