{"id":1116,"date":"2024-10-08T19:46:31","date_gmt":"2024-10-08T10:46:31","guid":{"rendered":"https:\/\/www.oakclinic-group.com\/wp\/ss_long\/"},"modified":"2024-12-07T22:02:42","modified_gmt":"2024-12-07T13:02:42","slug":"ss_long","status":"publish","type":"page","link":"https:\/\/www.oakclinic-group.com\/en\/funin\/ss_long\/","title":{"rendered":"Long-term Culture, Blastocyst Culture"},"content":{"rendered":"
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\r\n Long-term Culture, Blastocyst Culture\r\n <\/h1>\r\n\r\n
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\r\nSumiyoshi<\/a><\/span> Ginza<\/a><\/span>\r\n<\/p>\r\n<\/div>\r\n

\r\n<\/p>\r\n\r\n

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\r\n This is the method of transfer and cryopreservation of the developed embryo and blastocyst after OPU and culturing to Day 4, 7.\r\n \r\n We will be able to remove the embryos that stop splitting in the early term and strictly sort the good embryos.\r\n \r\n Early embryos and post-compaction (day 3-4) embryos and blastocysts require different nutrients, so during long-term culture, change the type of culture medium on day 3.\r\n \r\n Recently, the culture medium has been improved, and there is also a medium that can be cultured from early embryos to blastocysts.\r\n <\/p>\r\n

\r\n\"Long-term\r\n<\/p>\r\n<\/div>\r\n<\/div>\r\n

\r\n Flow of Long-term Culture\u30fbBlastocyst Culture\r\n <\/h2>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
\r\n Day 0\r\n <\/th>\r\n\r\n Oocyte Pick-Up\r\n \r\n Conventional IVF or\r\n \r\n ICSI Fertilization\r\n <\/td>\r\n\r\n\"Conventional\r\n<\/td>\r\n\r\n<\/td>\r\n\r\n<\/td>\r\n<\/tr>\r\n
\r\n Day 1\r\n <\/th>\r\n\r\n Pronuclear Period\r\n <\/td>\r\n\r\n\"Period\r\n<\/td>\r\n\r\n\"#\"\r\n<\/td>\r\n\r\n
\r\n Pyruvic acid is the source of\r\n \r\n energy of the early embryo\r\n <\/div>\r\n
\r\n\"#\"\r\n<\/div>\r\n
\r\n Culture Solution\r\n \r\n including much pyruvic acid\r\n <\/div>\r\n
\r\n\"#\"\r\n<\/div>\r\n
\r\n Organization close to the\r\n \r\n fallopian tubal fluid of people\r\n <\/div>\r\n<\/td>\r\n<\/tr>\r\n
\r\n Day 2\r\n <\/th>\r\n\r\n<\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n<\/th>\r\n\r\n Period of Cell Division\r\n <\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n Day 3\r\n <\/th>\r\n\r\n<\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n Day 4\r\n <\/th>\r\n\r\n Compaction\r\n \r\n

\r\n\"#\"\r\n<\/p>\r\n<\/td>\r\n

\r\n\"Period\r\n<\/td>\r\n\r\n\"#\"\r\n<\/td>\r\n\r\n
\r\n In the case of people\r\n \r\n it will become a compaction\r\n \r\n after 16cells and the energy\r\n \r\n source will change to glucose\r\n <\/div>\r\n
\r\n\"#\"\r\n<\/div>\r\n
\r\n Culture Solution including\r\n \r\n much glucose\r\n <\/div>\r\n
\r\n\"#\"\r\n<\/div>\r\n
\r\n Organization close to the uterus\r\n <\/div>\r\n<\/td>\r\n<\/tr>\r\n
\r\n Day 5\r\n <\/th>\r\n\r\n Blastocyst\r\n \r\n\r\n Early Blastocyst\r\n <\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n<\/th>\r\n\r\n Expanded Blastocyst\r\n <\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n<\/th>\r\n\r\n Hatched Blastocyst\r\n <\/td>\r\n\r\n\"Period\r\n<\/td>\r\n<\/tr>\r\n
\r\n<\/th>\r\n\r\n

\r\n\"#\"\r\n<\/p>\r\n<\/td>\r\n

\r\n<\/td>\r\n\r\n<\/td>\r\n<\/tr>\r\n
\r\n<\/th>\r\n\r\n

\r\n Implantation & Pregnancy\r\n <\/p>\r\n<\/td>\r\n

\r\n<\/td>\r\n\r\n<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/section>\r\n<\/div>\r\n<\/div>","protected":false},"excerpt":{"rendered":"Long-term Culture, Blastocyst Culture Sumiyoshi Ginza This is the method of transfer and cryopreservation of the developed embryo and blastocyst after OPU and culturing to Day 4, 7. We will be able to remove the embryos that stop splitting in the early term and strictly sort the good embryos. Early embryos and post-compaction (day 3-4) […]","protected":false},"author":2,"featured_media":0,"parent":1118,"menu_order":236,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_locale":"en_US","_original_post":"https:\/\/www.oakclinic-group.com\/wp\/?page_id=734","footnotes":""},"class_list":["post-1116","page","type-page","status-publish","hentry","en-US"],"_links":{"self":[{"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/pages\/1116","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/comments?post=1116"}],"version-history":[{"count":4,"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/pages\/1116\/revisions"}],"predecessor-version":[{"id":4666,"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/pages\/1116\/revisions\/4666"}],"up":[{"embeddable":true,"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/pages\/1118"}],"wp:attachment":[{"href":"https:\/\/www.oakclinic-group.com\/wp-json\/wp\/v2\/media?parent=1116"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}